摘要
目的建立雷公藤甲素脂质体中雷公藤甲素的含量测定方法。方法色谱柱μ-Bondapak C18(250mm×4.6mm,5μm);检测波长:219nm;流动相:甲醇-水-异丙醇(4:3:1);流速:1.0mL/min:柱温:30℃。结果雷公藤甲素与制剂中的辅料达到良好分离,雷公藤甲素在0.8—3.2μg范围内与峰面积有良好的线性关系(r=0.9998);平均回收率为97.65%,RSD为0.42%。结论此方法灵敏、准确、重现性好。
Objective To establish a method to determine the content of thyroxin in liposome of Tripterygium Wilfordii.Methods Chromatographic column ？ -Bondapak C 18 （250mm ＊ 4.6mm, 5？ m）; wavelength- testing： 219nm; mobilephase： methylaleohol - water - isopropanol （4： 3： 1 ） ; flow rate： 1.0ml/min; column temperature： 30℃. Results Thyroxin of tripterygium wilfordii and auxiliary ingredients in the phan＇naceutics separate satisfactorily. Thyroxin of tripterygium wilfordii has satisfactory linear relation with peak area between 0.8 - 3.2？ g （ r = 0. 9998 ） ; The average rate of recovery is 97.65%, RSD = 0.42%. Conclusion The method is sensitive, accurate with good reproduction.
出处
《陕西中医学院学报》
2006年第1期57-58,共2页
Journal of Shaanxi College of Traditional Chinese Medicine
关键词
HPLC法
雷公藤甲素脂质体
含量
method HPLC liposome of tripterygium wilfordii
content
