不同饲养环境、种属、佐剂及激发方法对小鼠哮喘炎症的影响

Impact of different feeding surroundings,strains,adjuvant and challenge methods on murine asthmatic inflammation

目的研究不同饲养环境、种属、佐剂及激发方法对小鼠哮喘样炎症的影响。方法应用普通级或清洁级的BALB/c和C57BL小鼠,以鸡卵白蛋白作过敏原,采用氢氧化铝和明矾佐剂及滴鼻和雾化的激发方式制备小鼠哮喘模型,进行支气管肺泡灌洗液(BALF)炎症细胞分类记数和观察细支气管周围的嗜酸性粒细胞(Eos)炎症。结果BALB/c和C57BL小鼠嗜酸性粒细胞炎症无差异,清洁级比普通级、明矾佐剂比氢氧化铝佐剂、多次的雾化比一次滴鼻的激发方式能更明显地诱导小鼠哮喘炎症。结论小鼠哮喘炎症程度受饲养环境、种属、佐剂及激发方法的影响,实验设计时应予充分的注意。

Objective To study the impact of different feeding surroundings, strains,adjuvants and challenge methods on murine asthmatic inflammation. Methods Conventional or clean BALB/c and C57BL mice were used to prepare asthma model by sensitization with chicken ovalbumin in aluminium hydroxide or alum adjuvant and intranasal or aerosol challenge. Differential inflammatory cell populations in bronchoalveolar lavage fluid and the number of eosinophils within the inflamed peribronchial region were analyzed. Results There was no significant difference in eosinophil inflammation between BALB/c and C57BL asthma-like mice. More obvious asthmatic inflammation was observed in clean mouse with alum adjuvant and aerosol challenge than in conventional with aluminium hydroxide and intranasal challenge. Conclusion The asthmatic inflammatory degree of mice was influenced by feeding surroundings, strains, adjuvant and challenge methods, which should be considered in experimental design.