胚胎因素对体外子宫内膜整合素β3的调节

Embryonic regulation of intergrin β3 in mice endometrial epithelial cell in vitro

目的:研究胚胎加入前后体外子宫内膜上皮细胞整合素β3的变化。方法:用已建立的子宫内膜体外培养三维模型,RT-PCR半定量法和免疫印迹法测定胚胎加入前后子宫内膜上皮细胞整合索β3亚基的表达。结果:胚胎加入后整合素β3 mRNA的表达量(1．021±0．022)与对照组(0．434±0．154)相比,表达升高(P<0．05)。整合β3蛋白的表达量 (126．83±8．27)与对照组(69．90±6．16)相比,明显升高(P<0．01)。结论:胚胎可促进子宫内膜整合素β3的表达,在子宫内膜容受性形成中具有促进作用。

Objective：To study the effect of embryos on the regulation of integrin β3 in cultured mice endometrial cells with a three-dimensional model. Methods： The mice blastocysts on day 4 of pregnany were cultured on a three-dlmensional endometrium in vitro,the level of integrin β3 mRNA was measured with RT-PCR and prtein measured with inununo-blot method. Results：The level of in- tegrin β3 mRNA and protein expression was significantly increased by embryos （ 1. 021 ±0. 022 vs 0. 434 ±0. 154,126. 83 ±8.27 vs 69. 90±6. 16）（P 〈0. 05 ,P 〈0. 01 ）. Conclusion：The blastocysts could up-regtdate integrin β3 whlch can induced the uterine receptivity.