灯盏花素聚丙烯酸酯纳米球的制备及载药机理探讨

Mechanism of breviscapine entrapped by polymethacrylate nanospheres

目的:制备灯盏花素聚丙烯酸酯纳米球,研究聚丙烯酸酯纳米球与灯盏花素之间的载药机理。方法:采用微乳液聚合法制备聚丙烯酸酯纳米球,表面活性剂为十二烷基硫酸钠(SDS),助表面活性剂为丁醇,单体为甲基丙烯酸(MAA)、甲基丙烯酸丁酯(BMA),交联剂为三羟甲基丙烷三甲基丙烯酸酯(TRIM),引发剂为偶氮二异丁乙腈(AIBN);灯盏花素的载药分别采用在聚合反应之前和之后加入二种方式。结果:制备的聚丙烯酸酯纳米球大小在50nm左右,表面电势ζ为-27.2mv,负载灯盏花素以后纳米球电位增大。灯盏花素包封到聚丙烯酸酯纳米球上时,其载药量与加入药量呈线性相关;灯盏花素负载到聚丙烯酸酯纳米球上时,载药量呈层状增加。结论:通过微乳液聚合制备的聚丙烯酸酯纳米球可用于包封脂溶性中药成分。

AIM ： To prepare polymethacrylate nanospheres and study the mechanism of breviscapine entrapped by polymethacrylate nanospheres. METHODS： Polymethacrylate nanospheres were prepared by microemulsion polymerization with SDS as surfactant, n-butanol as cosurfactant, MAA and BMA as monomer, TRIM as cross-linker and AIBN as initiator. Breviscapine added would be divided into two ways：prior to polymerization （encapsula- tion） and after polymerization （sorption） , respectively. RESULTS ： The size of the nanospheres was found to be 50nm by measuring with TEM. The ~ potential of the nanospheres was - 27.2 mv and it was increased after breviscapine being entrapped. The drug content entrapped in nanospheres was proportional to the drugs amount added in encapsulation method while the drug content in nanospheres was increased step by step in sorption method. CONCLUSION： The polymethacrylate nanospheres prepared by microemulsion polymerization could be applied to encapsulate hydrophobic traditional Chinese medicine extracts.