大孔吸附树脂纯化山楂叶总黄酮工艺研究

Purification process of total flavonoids of Crataegus leaves with macroreticular resin

目的:研究HPD-600大孔树脂纯化山楂叶总黄酮的工艺条件及参数。方法:采用正交实验法,以固形物中总黄酮含量为考察指标,对影响黄酮提取工艺的因素进行了研究。结果:在实验设计的影响因素中,上柱粗提液浓度和洗脱液组成对纯化效果有显著影响。结论:经微波辅助提取并通过大孔吸附树脂纯化后,山楂叶产品中总黄酮含量可达80.75,芦丁含量达1.38,说明本法纯化山楂黄酮是可行的。

AIM ： To study the technological parameters of the purification process of total flavonoids of Crataegus leaves with HPD-600 macroreticular resin. METHODS： Using orthogonal experiments,the adsorptive characteristics and elutive parameters of the process were studied by taking the elutive effects and purification ratio of total flavonoids of Crataegus leaves. RESULTS ： Two significant factors which were the concentration of crude flavonoids solvent and elutant component were observed. CONCLUSION： The microwave-assisted extraction and purification process with macroreticular resin is employed, the content of total flavonoids is 80.75%, the rutin content is 1.38%, The macroreticular resin can be used in the purification of flavonoids of Crataegus leaves.