大黄虫丸对改良阿霉素肾病肾硬化大鼠模型作用的实验研究

Protective effect of Dahuang Zhechong Pills on the improved driamycin nephropathy mouse models

目的:研究大黄虫丸延缓慢性肾小球疾病进展的作用及机理。方法:采用切除右肾,尾静脉注射阿霉素及口服高脂饮食的复合方法塑造大鼠阿霉素肾病肾硬化模型,将实验动物分为正常对照组、肾病模型组、雷公藤多苷组、大黄虫丸小剂量组和大剂量组,分别喂予生理盐水、雷公藤多苷15mg/kg、大黄虫丸0.75g/kg、大黄蜇虫丸1.5g/kg,对各组实验动物的血尿生化及肾脏病理组织变化进行比较研究。结果:大黄虫丸给药后可使肾病大鼠体重增加,摄食量明显增多,明显降低肾病大鼠尿蛋白量,明显降低肾病模型组胆固醇(CHO)和甘油三酯(TG)值,同时可升高血总蛋白(TP)和白蛋白(ALB)含量,高剂量组并可降低血尿素氮(BUN)、血肌酐(Scr)水平,优于雷公藤多苷组。肾组织病理变化观察发现:大黄庶虫虫丸治疗组均表现出减轻炎性细胞浸润、抑制系膜细胞增生和减轻间质纤维化的作用,并且高剂量组改善作用更明显。结论:大黄虫丸具有调脂,减轻炎细胞浸润、抑制系膜细胞增生和减轻间质维化,能防止肾小球硬化的多方面作用。

AIM： To investigate the preventive effect of Dahuang Zhechong Pills （DHZCP）on the progression of chronic glomerular disease. METHODS： The mouse adriamycin nephropathy models were established by the combined methods including right renal resection, tail intravenous infusion of adriamycin and feed by hyperlipid food. All the animals were randomly divided into normal control group ,nephropathy model group ,tripterygium wilfordii（TW）group,DHZCP small dosage group and large dosage group. The blood and urine laboratory data and renal pathology of the mice were studied respectively. RESULTS ： The weight and the food intake of mice were increased significantly in DHZCP groups compared with the nephropathy model group （P 〈 0.05 ）. After 10 weeks＂ observation, the level of serum CHO and TG decreased in the DHZCP groups compared with nephropathy model group （ P 〈 0.05）. DHZCP could also reduce proteinuria. The serum total protein and albumin increased while the level of BUN and SCr decreased in DHZCP groups compared with TW group （ P 〈 0.05 ）. Pathology study showed that the infusion of the inflammation cells ,the proliferation of mesangial cells and the fibrosis of the renal interstitium were reduced in DHZCP groups, especially in the large dosage group. CONCLUSION ： DHZCP could regulate the disorder of the fat metabolism, anti inflammation and retard renal fibrosis.