流式细胞术在急性白血病细胞免疫表型分析中的价值

Flow cytometry analysis of actue leukemia immunophenotyping

目的研究应用流式细胞技术对急性白血病进行免疫表型分析,为临床治疗和预后提供有价值的信息。方法运用双色免疫荧光抗体标记患者骨髓或外周血单个核细胞,用流式细胞仪分析白血病细胞膜免疫表型。结果分析75例急性白血病患者中,急性T淋巴细胞白血病(T-ALL)患者12例,占16.0%;急性B淋巴细胞白血病(B-ALL)患者24例,占32.0%;急性髓细胞白血病(AML)患者37例,占49.3%。混合型急性HALL/B有2例,占2.6%。ALL患者除均表达淋系抗原外,同时伴有髓系统抗原的表达;AML患者除均表达髓系抗原外,少部分同时伴有淋系抗原的表达。AML细胞表面抗原表达百分率成人明显高于儿童(73%/27%)。结论流式细胞技术能准确地分析并鉴别出急性白血病各型别,是白血病诊断分型的重要手段之一。

Objective To analyse actue leukemia immunophenotyping by flow cytornetry and provide valuable information for clinical treatment and diagnoses. Methods Apply two color immunofluorescece antibody marking single cell of patients＇ oute blood or marrow, analyse immunophenotyping of these marked leucocyte by flow eytcmetry. Results 76 actue leukemia cases were analysed, 12 cades were T- ALL occuping 15.8% ,22 cases were B- AlL occuping 28.9% ,36 cases were AML occuping 47.4% and 6 cases were HAIM/L. Occuping 7.9% .Patients with ALL expressed both lymphocyte antigen and myeloid antigen. Patients with AML expressed myeloid antigen, a small part cases expressed lymphocyte antigen at the same time. Patients with HAML/L all expressed lymphocyte antigen and cmeloid antigen. Expression rate of AML＇ cell antigen in adult is remarkably higher than that in children（75 %/27 % ）. Conclusion Flow cytometry can analysis and distinguish all types of actue leukemia exeatly, it is an important method for leukemia＇ diagnoses and type defining.