慢性乙型肝炎患者外周血CD45RA+,CD45RO+T淋巴细胞亚群的特点及临床意义

Characteristics of the Peripheral Blood CD45RA+,CD45RO+T Lymphocyte Subsets in the Patients with Chronic Hepatitis B and Its Clinical Significance

目的 探讨慢性乙型肝炎患者外周血CD4＋CD45RA＋，CD4＋CD45RO＋，CD8＋CD45RA＋和CD8＋CD45RO＋T淋巴细胞亚群的特点及及其与肝病病情的关系．方法 采集46例轻中度慢性乙型肝炎患者、58例重度慢性乙型肝炎患者和30例健康人的外周抗凝血，应用流式细胞技术三色荧光分析法对其外周血中CD4＋CD45RA＋，CD4＋CD45RO＋，CD8＋CD45RA＋和CD8＋CD45RO＋T淋巴细胞亚群进行检测，结果轻中、重度慢性乙型肝楚患者与正常人相比．其外周血中CD4＋，CD8＋T细胞均无明显改变；CD8＋CD45RA＋T细胞均明显降低，CD8＋CD45RO＋T细胞均明显增高，而CD4＋CD45RA＋，CD4＋CD45RO＋T细胞均无明显改变；重度慢性乙型肝炎患者与轻中度慢性乙型肝炎患者相比，CD8＋CD45RA＋T细胞明显降低（P〈0．05），CD8＋CD45RO＋T细胞明显升高（P〈0．05）．结论乙型肝炎慢性化过程中，CD8＋CD45RO＋T细胞起重要作用且与慢性乙型肝炎患者病情的进展呈正相关；检测CD4＋CD45RA＋．CD4＋CD45RO＋．CD8＋CD45RA＋和CD8＋CD45RO＋T淋巴细胞亚群比检测CD4＋和CD8＋T细胞亚群能使我们更加正确、充分、全面地了解慢性乙型肝炎的发病机制和预后，从而有效地指导临床治疗。

Objective To discuss the characteristics of CD4 ＋ CD45RA ＋ , CD4 ＋ CD45RO ＋ ,CD8 ＋ CD45RA ＋ and CD8 ＋ CD45RO ＋ T lymphocyte subsets in the peripheral blood of the patients with chronic hepatitis B and to explore their relations with the disease state. Methods The peripheral blood were collected from 104 patients with chronic hepatitis B and 30 healthy individuals, then CD4 ＋ CD45RA ＋ , CD4 ＋ CD45RO ＋ , CD8 ＋ CD45RA ＋ and CD8 ＋ CD45RO ＋ T lymphocyte subsets were detected by flow eytometry with three-color fluorescence technology. Results To compare with the control, the percent of CD8 ＋ CD45RA ＋ T lymphocyte in patients with mild and severe grad CHB decreased markedly, the percent of CD8 ＋ CLMSRO ＋ T lymphocyte increased markedly, CD4 ＋, CD8 ＋, CD4 ＋ CD45RA ＋ and CD4 ＋ CD45RO ＋ T lymphocyte did not change obviously. To compare with the mild grade CHB, the percent of CD8 ＋ CD45RA ＋ T lymphocyte in patients with severe grade CHB decreased obviously （P 〈 0.05）, however, the percent of CD8 ＋ CD45RO ＋ T lymphocyte increased obviously （ P 〈 0.05）. Conclusion CD8 ＋ CD45RO ＋ T lymphoeyte plays an important role in the pathogenic mechanism of CHB and the percent of it exhibites positive correlation with the disease state.