白藜芦醇对卡介苗和脂多糖所致小鼠肝损伤的保护作用

Protective effect of resveratrol on BCG+LPS induced acute hepatic injury in mice

目的研究白藜芦醇对卡介苗和脂多糖所致小鼠性肝损伤的保护作用。方法60只小鼠随机分为正常组、模型组、白藜芦醇大、中、小剂量给药组、阳性对照组等6个组。白藜芦醇给药组按30mg/kg、15mg/kg、7.5mg/kg剂量,阳性对照组以联苯双酯150mg/kg灌胃,正常组、模型组以等体积生理盐水灌胃,共10d。模型组和给药组尾iv注射卡介苗和脂多糖,正常组尾iv等体积生理盐水。尾iv脂多糖16h后,摘眼球取血,用比色分析法测定小鼠血清门冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)的活性,血清、组织匀浆超氧化歧化酶(SOD)、一氧化氮(NO)的活性及丙二醛(MDA)的含量。结果白藜芦醇显著降低卡介苗和脂多糖所致急性肝损伤小鼠血清ALT的水平,可明显升高肝组织SOD活性和血清NO含量,降低肝组织MDA含量。结论白藜芦醇对卡介苗和脂多糖所致小鼠急性肝损伤有较好的保护作用。

Objective To study the protective effect of reseratml on BCG ＋ LPS induced acute hepatic injury in mice. Methods 60 mice were randomly divided into six groups, namely normal group, model group, low, middle and high dosage resverratrol groups and a positive control group. The doses of 30mg/kg, 15mg/kg and 7.5mg/kg were injected into the stomach of the mice in resverratrol groups, the dose of 150mg/kg of biphenyldiearboxylate was injected into the stomach of mice in positive control group and the same volume of saline was injected into he stomach of mice in normal and model groups for a period of 10 days. The mice in model group and three resverratrol groups were intravenously given BCG - LPS through tail; while the mice in normal group were intra- venously given the same volume of saline through tail. The eye balls of mice were removed and blood colleeted 16 hours after intra- venous infection of LPS, then the activity of serum aspartate transminase, alanine transminase, superoxide dismutase, nitrogen monoxide and the contents of serum malondialdehyde were measured by colorimetry. Results Resverratrol can significantly reduce BCG ＋ LPS induced acute hepatic lesion to mice by decreasing serum aspartate transminase and alanine transminase and enhancing the activities of superoxide dismutase and the content of nitrogen monoxide in liver tissue and then reducing the content of malondialdehyde in liver tissue. Conclusion Resverratrol possesses the effect of preventing acute hepatic lesion to mice induced by BCG ＋ LPS.