精子顶体酶活性与精液有关参数的研究

The investigation of the relationship between activity of human sperm acrosin and semen-related parameters

目的分析精子顶体酶活性与精液有关参数变化,探讨精子顶体酶活性对男性不育的影响。方法对214例精液标本作精子顶体酶活性、弹性硬蛋白酶、果糖、α-葡萄糖苷酶、锌、酸性磷酸酶、精子尾部低渗肿胀试验检测,同时用WLJY-9000伟力彩色精子质量检测仪作精液分析。以精子顶体酶活性正常的精液作为对照组,以精子顶体酶活性异常的精液作为观察组,并对两组参数进行比较。结果214份精液标本,精子顶体酶活性正常111例,异常103例。对照组在精子密度、精子活动率、a+b级精子百分率、精子尾部低渗肿胀试验均明显优于观察组(P<0.001),弹性硬蛋白酶、果糖、α-葡萄糖苷酶均明显优于观察组(P<0.05),精液量、锌、酸性磷酸酶无差别(P>0.05)。结论精子顶体酶活性与精液有关参数关系密切,精子顶体酶活性降低将能引起男性不育。

Objective To analyze the activity of human sperm arosin and the variation of semen - related parameters, and study the effect of sperm acrosin activity on human infertility. Methods The activity of human sperm acrosin, PMN - Elastase, Fruclose, α-glucosidas-e, Zinc, Acid phosphatase and HOS in 214 cases were tested determined. The sperm was analyzed by using WLJY - 9000 WeiLi colorful spermic mass device. The normal sperm arosin was taken as control and the sperm arosin of abnormal activity was taken as observation group. Results Out of the 214 sperm samples normal sperm activity was observed in 111 samples and abnormal perm activity was observed in 103 samples. In comparison with the observation group, the parameters of sperm density, motile sperm rate, grade a ＋ b sperm percent and HOS in the control group were superior to the observation one （P 〈 0.01 ）. significant difference in PMN- Elastase was also observed （P 〈 0.02）. The parameters of fructose and α-glucosidase in the observation group were also significant as compared with the control （P 〈 0.05） and no significant differences in sperm volume,zinc and acid phosphatase concentrations were observed between the two groups （P 〉 0.05）. Conclusion The activity of sperm arosin is closely related with the sperm components and the variation of the sperm component will reduce the activity of sperm arosin and resulting in infertility in males.