摘要
目的 研究畜禽肉及内脏类样品中己烯雌酚的高效液相色谱测定方法。方法 用甲醇二次提取畜禽内及内脏中己烯雌酚,以AgilentSB-C18色谱柱分离,二极管阵列检测器(DAD)高效液相色谱法测定舍量。结果 按常规取样5g时方法最小检出浓度0.24mg/kg;加标回收率分别为98.7%-104.3%和99.6%-101.7%;精密度RSD〈5%;范围0.06-1.20ug/ml,r=0.9996。结论 用HPLC法测定己烯雌酚简便快捷,有较好的精密度和准确度,较适用于畜禽肉及内脏中己烯雌酚的测定。
Objective To explore the methods of determination of diethylstilbestrol in meat and viscera of domesticated poultry and animals by high performance liquid phase chromatography ( HPLPC ). Methods Diethylstilbestrol was extracted by using methanol and separated with Agilent SB-C18 column and the content was determined by diode array detector (DAD), Results The minimum detection concentration was 0.24mg/kg if the quantity of sample was 5g. Tile recovery rate of added sample was 98.7% ~ 104.3% aad 99.6% - 101.7 %. The RSD was 〈 5% , There showed a good linear relationship in the range of 0.06~1. 20μml (r = 0.9996), Conclusion HPLPC is a rapid, simple and accurate method for determination of diethylstilbestrol.
出处
《中国热带医学》
CAS
2006年第2期328-329,共2页
China Tropical Medicine