高效液相色谱法测定硫酸奈替米星注射液的含量

Determination of content of netilmicin sulfate injection by using high performance liquid phase chromatography.

目的采用高效液相色谱法测定硫酸奈替米星及注射液的含量。方法以μ-Diamonsil—C18为分析柱，流动相为1．5％的磷酸二氢钾-乙睛，流速为1．0ml／min，检测渡长为205nm。含量测定用峰面积外标法。结果硫酸奈替米星在0．1—1．6mg／ml浓度范围内呈现良好线性关系，r=0．9998．平均回收率为99．5％，RSD=0．4％（n=9）。结论本方法操作简便、快速，结果准确可靠，重现性好，均能很好地满足质量控制的要求，为开辟氨基糖苷类抗生素含量测定的仪器提供了一定借鉴。

Ohjective To determine the content of netilmicin by using high performance liquid chromatography. Methods The analytical column was u - Diamonsil- C18, the mobilephase was 1. 5 % two - kydrogen kalium of phosphoric acid（pH value was 2.5） -acetonitrile （500：100） with 1.0ml/min of flow rate, the detective Wavelength was 205 nm. External standard method was used. Results The stanadard curve of netilmicin sulfate was linear in the concentration of 5-5g/L （r = 0.9998）, the average recovery was 99.5 %, RSD = 0.4 % （ n = 9）. Conclusion High performance liquid chromatography is a simple, radid and accurate method for determinating the content of netilmicin sulfate Injection.