鞭枝藻与其突变体提取液对人膀胱癌细胞生长的影响

The Influence of Extracts of Mastigocladus laminosus and Its Mutant for Growth of 9T24C Tumor Cells

本文以EMS(乙基甲烷磺酸盐,Sigma)诱变嗜温的层理鞭枝藻(Mastigocladus laminosus),并经9T24C人膀胱癌细胞生长选择获得突变体。在0.3％FBS(胎牛血清)+DME/F12培养液中加入100μg/ml层理鞭枝藻突变体提取液可促进9T24C癌细胞数在6天中增长3.1倍,而在同样条件下野生型层理鞭枝藻的提取液则对9T24C癌细胞无明显刺激生长作用。这种未知的刺激癌细胞生长的活性物质分子量>3500。

A mutant of thermophilic Mastigocladus laminosus, a blue-green algae, has been obtained by mutantion of EMS ( Ethyl Methanesulfonate, Sigma ) and selection of growth on 9T24C tumor cells.Extracts of mutant promote the growth of 9T24C cells in DME/F12 medium supplemented with 0.3% FBS (Fetal Bovine Serum) . The cell number of 9T24C cells increased 3.1-folds in six days when 100 fig/ml extract was added into medium. The molecular weight of unknown active component more than 3500.