摘要
用阳离子交换柱的HPLC法同时测定注射用氨苄西林钠/氯唑西林钠中氨苄西林和氯唑西林的含量。色谱条件:采用Hypersil SCX柱,磷酸盐缓冲液(0.01mol/L磷酸氢二铵溶液,用磷酸调节pH值6.0):乙腈(90:10)为流动相,检测波长225nm。氨苄西林和氯唑西林分别在45-134和44~131μg/ml范围内呈良好的线性关系,日内RSD分别为0.5%和0.6%,回收率分别为99.9%和100.0%,含量测定结果与国家药品标准方法所得结果相符。
A newly proposed HPLC method for simultaneous content determination of ampicillin and cloxacillin in a pharmaceutical formulation for injection was developed. The chromatographic condition was as follows, column: Hypersil SCX; mobile phase: phosphate buffer (0.01mol/L dibasic ammonium phosphate, adjusted to pH 6.0 with phosphoric acid) : acetonitrile (90 : 10); detection wavelength: 225nm. The standard curves were linear within the range of 45-134 and 44-131μg/ml for ampicillin and cloxacillin respectively. The precisions in a day (RSD) were 0.5% and 0.6% respectively. The assay results complied with those obtained by using the national standard method.
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
2006年第1期56-58,共3页
Chinese Journal of Antibiotics