局灶亚低温治疗创伤性脑损伤的最佳灌流温度

The study of optimal perfusion temperature of focal hypothermia in rats with traumatic brain injury

目的探讨创伤性脑损伤局灶亚低温治疗的最佳灌流温度。方法采用Feeney自由落体改良模型,设定对照组、颅脑损伤组、全身亚低温组、局灶亚低温组(0℃、10℃、20℃、25℃组)。取伤灶区脑组织分别检测其水、Na+、K+含量,同时观察其病理改变。结果10℃、20℃、25℃组神经元损伤数显著少于全身亚低温组(P<0.01)。25℃组水含量明显低于全身亚低温组(P<0.05),20℃、25℃组Na+含量均显著低于全身亚低温组(P<0.01),K+含量均显著高于全身亚低温组(P<0.01)。结论25℃水温是创伤性脑损伤局灶亚低温治疗的最佳灌流温度。

Objective To find the optimal perfusion temperature of focal hypothermia in rats with traumatic brain injury （TBI）. Methods Modified Feeney＇s free-falling model was used in the study. Forty-nine male Sprague-Dawley （SD） rats were randomly divided into seven groups： TBI group, systemic hypothermia group, focal hypothermia groups （including 0℃, 10℃, 20℃ or 25℃ subgroups, respectively） and control graup. The contents of total sodium, potassium and water of traumatic brain were measured, and pathological changes were examined in the seven groups. Results The damaged neurons were significantly fewer in focal hypothermia groups （including 10℃, 20℃ or 25 ℃ subgroups, respectively） than those in systemic hypothermia group at 72 hours after TBI （ P 〈 0.01）. The content of water was significantly lower in focal hypothermia group （ 25℃ subgroup） than that in systemic hypothermia group after TBI （ P 〈 0.05）. The content of total sodium was significantly lower in focal hypothermia groups （including 20 ℃ or 25 ℃ subgroups） than that in systemic hypothermia group （P 〈 0.01 ）, and the content of total potassium was significantly higher in focal hypothermia groups （including 20℃ or 25℃ subgroups） than that in systemic hypothermia group （P 〈 0.01）. Conclusion The water temperature of 25℃ is the optimal perfusion temperature of focal hypothermia in rats following TBI.