5-氟尿嘧啶对脓毒性休克大鼠的保护作用

Protective effect of 5-fluorouracil in rats with septic shock

目的观察5氟尿嘧啶(5FU)对脓毒性休克大鼠预后的影响。方法将30只Wistar大鼠随机分成对照组、脓毒性休克组和5FU治疗组。实验操作6h后处死大鼠,观察各组大鼠的病死率和腹水率,称取肺组织湿重,收集肺组织标本做病理学检查,评估肺组织损伤程度。结果与脓毒性休克组相比,5FU组大鼠病死率和腹水率显著减少,肺湿重和肺损伤评分明显好转。结论5FU对脓毒性休克大鼠有保护作用。

Objective To observe the effects of 5-fluorouracil （5-FU） on prognosis of septie shock in rats. Methods Thirty female Wistar rats was randomly divided into three groups： control group, septic shock group, and 5-FU group. The septic shock model was established by peritoneal injection of 1.8× 10^9cfu/ml E. coli at the volume of 1 ml/100 g weight. The 5-FU group rats were treated intraperitonealy with 5-FU 50 mg/kg weight at 40 minutes after the septic shock model was established. All rats were killed at 6 hours after operation. The mortality, the ascites rate, the lung tissue wet weight and the lung injury score （LIS） were measured. Results The mortality, the aseites rate, the lung tissue wet weight and LIS of the septic shock group was 60%, 90%, （1.55± 0.17） g, and （8.00 ± 2.54）, respectively. In 5-FU group, these parameters were 0, 10%, （1.36 ± 0.14） g, and （5.20 ± 2.39）, respectively. Compared with septic shock group, the mortality and the ascites rate of 5-FU group were significantly decreased, and the lung tissue wet weight and LIS were improved. Conclusion 5-FU can decrease the ascites rate, relieve inflammation and tissue damage.