皱纹盘鲍经诱导后血淋巴中一些因子变化的研究

STUDY ON THE CHANGES OF SOME FACTORS IN HAEMOLYMPH OF INDUCED HALIOTIS DISCUS HANNAI

于1995年11月在山东荣城大鱼岛鲍鱼养殖厂采集皱纹盘鲍，以大肠杆菌、弧菌为诱导物，将鲍分为三组分别进行注射，在注射后的5，18，32，50h分别取鲍血淋巴，测定其抗菌、溶菌、酚氧化酶和超氧化物歧化酶活力，并研究注射组各项活力的变化规律。实验结果表明，适当的刺激可增加皱纹盘鲍的抗病能力。

Speciments of Haliotis discus Hannai were collected from Rongcheng,Shandong Province in November 1995. The levels of antibacterial, acteriolytic,phenoloxidase and superoxide dismutase (SOD) activities and the blood cell number in the haemolymph of Haliotis discus Hannai were tested at 5, 18, 32, 50h after injection with filtered seawater (conirolled group), with E coli, and with Vibrio sp.The results showed that those infected with E. coli had significant elevations in bacteriolytic activity in the serum at 32h after injection: those infected with Vibrio sp.had the highest level at 5h after injection. Ths proved that the two kinds of bacteria could induee elevations in bpeteriolytic aCtivity in the haemolymph of Haliotis discus Hannai. It is suggested that infeetion with Vibrio sp. and E. coli could induce elevations in antibacterial activity and blood cell number, but that the effeCt of infection with the two kinds of bacteria on phenoloxidase and SOD activities was not obvious. All these proved that proper stimulus can increase the immune protency of H. discus Hannai.