摘要
根据GenBank中报道的NDV融合蛋白基因(F)序列,设计了一对特异性引物,用该引物对所分离的LN-SN株进行了RT-PCR扩增;将扩增得到的PCR片段纯化后与pGEM-T连接得到重组质粒pGEM-F,用于核苷酸序列测定。结果该基因ORF长1662bp,编码553个氨基酸;将LN-SN毒株与GenBank中已报道的NDV毒株进行比较,F基因核苷酸序列的同源性在83.1%～98.9%之间,推导的F蛋白氨基酸序列的同源性在88.1%～99.5%之间。
According to fusion protein gene sequence of Newcasde Disease virus strain reported by GenBank database, a pair of specific primers was designed and used to amplify F gene of LN-SN strain. The positive PCR product was purified and ligatured with pGEM-% The correct positive recombinant was used for sequencing. The open reading frame length of F gene of LN-SN strain was 1662bp and encoded 553 amino acids. The homology of nucleic acid and amino acid among the strains of NDV accessed by GenBank database ranged from 83.1 to 98,9% and from 88.1 to 99.5%.
出处
《畜禽业》
2006年第1期22-25,共4页
Livestock and Poultry Industry
关键词
新城疲病毒
融合蛋白基因
遗传变异
Newcastle Disease virus
fusion protein gene
Heredity and Variation
