反相高效液相色谱法测定人血浆中二甲胺四环素浓度

Determination of Minocycline in Human Plasma by RP-HPLC

目的:建立以反相高效液相色谱法测定人血浆中二甲胺四环素浓度的方法。方法:色谱柱为C18,流动相为乙腈-水-三氟乙酸(15∶85∶0.1),内标为土霉素,检测波长为350nm,血样在pH=6.5缓冲液条件下用乙酸乙酯提取,以二甲胺四环素与内标的峰面积比进行定量。结果:二甲胺四环素检测浓度线性范围为0.05～8μg/ml(r=0.9999),最低检测浓度为0.02μg/ml,平均回收率为101.89%,日内、日间相对标准差均小于5%。结论:本方法简便、快速、灵敏,可用于二甲胺四环素药动学研究。

OBJECTIVE ： To develop a RP HPLC method for determination of minocycline concentration in human plasma. METHODS： Chromatographic separation has been achieved on C18 column with acetonitrile- H20 TFA（15 ： 85 ： 0.1） as the mobile phase, and oxytetraeyeline as internal standard. The detection wavelength was 350nm. The minocycline was extracted from buffered plasma（pH=6.5）by ethyl- acetate, and quantified by the ratio of minocycline peak area to that of internal standard. RESULTS ：The linear range of minocycline detection concentration was 0.05-8μg/ml （r =0.9 999）. The minimum detection concentration was 0.02μg/ml with an average recovery of 101.89% . The inter and intra-day RSD were both less than 5% CONCLUSION ：The present method is simple, rapid and accurate for determination of minocycline in human plasma.