紫外分光光度法测定特美肤涂剂中丙酸氯倍他索的含量

Content Determination of Clobetasol Propionate in Temeifu Pigmentum by UV Spectrophotometry

目的:建立测定特美肤涂剂中丙酸氯倍他索含量的快速方法。方法:采用紫外分光光度法,检测波长为240nm。结果:丙酸氯倍他索检测浓度在12～18μg/ml范围内与吸收度线性关系良好;日内、日间平均回收率分别为99.87%(RSD=1.21%)、99.79%(RSD=1.01%)。结论:本方法简便、快速、准确,尤其适用于未购置高效液相色谱仪的基层医院。

OBJECTIVE; To establish a method for quick content determination of clobetasol propionate in temeifu pigmentum.METHODS：UV spectrophotometry was used, and the determination was performed at the wavelength of 240nm.RESULTS：The detectable concentration of clobetasol propionate have a good linear relationship with its absorbability in the range of 12-18μg/ ml.The inter-day and intra-day average recoveries were 99.87% （RSD=1.21% ） and 99.79% （RSD = 1.01% ）,respectively.CONCLUSIONS：The present method is rapid and accurate, which can be widely used in small hospitals not equipped with high performance liquid chromatography.