山茱萸环烯醚萜总苷对实验性糖尿病心脏病变的保护作用

Protecting Effect of Total Iridoid Glycoside in Fructus Corni Officinalis on Experimental Diabetes Models with Heart Disease

目的研究山茱萸环烯醚萜总苷(ICO)对实验性糖尿病心脏病变的保护作用。方法以高脂饲养结合STZ腹腔注射造模。于造模后2周分别灌胃给予降糖通脉(JTTM)胶囊(0.8 g/kg)I、CO(0.05、0.10、.2 g/kg)治疗,连续16周。检测空腹血糖、血清NO、NOS、血浆ET含量,取心脏计算心指数,并采用光镜观察其形态学改变。结果模型组大鼠空腹血糖升高,血清NO、NOS水平明显降低,血浆ET水平明显升高,心指数增加(P<0.05,P<0.01)。光镜检查显示模型大鼠心肌肥大,伴程度不等的间质水肿和组织细胞增生;心内膜或心包膜有轻度炎症及纤维化。应用ICO及JTTM后,糖尿病大鼠血糖降低,血清NO、NOS水平升高,血浆ET水平降低,心指数降低,心肌肥大程度减轻。结论ICO对糖尿病心脏病变有一定的保护作用。

OBJECTIVE To investigate into the protecting effect of total iridoid glycoside in Fructus Corni Officinalis on experimental diabetes models with heart disease. METHOD Models were made with high - fat diet and STZ injection. Two weeks later, Jiangtang Tongmai Capsule （0.8 g/kg） and ICO （0.05, 0.1, 0.2 g/kg） were used in the treatment for 16 weeks. RFSULT In the model group, fasting blood sugar of the rats increased, the levels of serum NO and NOS decreased, plasma ET level rose markedly, heart indexes increased （ P 〈 0.05, P 〈 0.01 ）, overgrowth of the heart muscle occurred as revealed by optical microscope, varying degrees of matrix edema and tissue cell hyperplasia appeared, and there were mild inflammation and fibrosis in the endocardium and pericardium. After the application of ICO and Jiangtang Tongmai Capsule, the symptoms were relieved. CONCLUSION ICO has certain protecting effect on diabetes with heart disease.