摘要
目的:分析不同药用菊花栽培类型的遗传差异。方法:采用2%CTAB法进行药用菊花的DNA提取,并用RAPD技术对22个类型药用菊花种质资源的DNA进行检测,采用NTSYS软件对扩增结果进行统计和聚类。结果:筛选得到的26个引物共得到了233条扩增DNA片断,其中89.7%的扩增条带表现出多态性,每个引物平均可扩增得到8.04条多态性片断。聚类分析结果表明利用RAPD技术可将全部供试材料区分开。结论:药用菊花种质资源在分子水平上确实存在较大差异;药用菊花栽培类型间的差异与环境因素有关,但更大程度上由其遗传因素决定。
Objective： To investigate the genetic diversity of Chrysanthemum morifolium at the level of molecular biology. Method： The total genomic DNA was extracted from medicinal chrysanthemums by 2% CrAB method. And the genetic diversity of 22℃. morifolium accessions was tested by RAPD marks. The NTSYS software was used to analyze the marks. Result： 26 10-mer arbitrary primers were found to acquire polymophic results. A total of 233 bands were amplified, of which 89.7% bands were found to be polymophic. 8.04 polymophic bands were amplified by each primer on the average. The results of cluster analysis by using UPGMA method showed that all the tested accessions could be differentiated by RAPD marks. Conclusion： There actually existed much genetic diversity at the molecular level among the germplasm resources of C. morifolium. RAPD marks could be effective tools to construct DNA fingerprintings of C. morifolium. The differences between the tested chrysanthemums are related to the environments. However, it was affected by genetic facters more significantly.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2006年第1期18-21,共4页
China Journal of Chinese Materia Medica
基金
国家科技攻关计划项目(2004BA721A202001BA701A24-0999-929-01-16)
江苏省自然科学基金资助项目(BK2000020)
中央级科研院所科技基础性工作专项项目(中药材标准--菊花)
关键词
菊花
RAPD
遗传多样性
聚类分析
Chrysanthemum morifolium
RAPD
genetic diversity
cluster analysis
