垂盆草中三萜类成分δ-香树酯酮的含量测定

Determination of triterpene δ-amyrone in Sedum sarmentosum

目的：用HPLC-ELSD测定中药垂盆草中三萜类成分δ-香树酯酮的含量，以期为中药垂盆草的质量控制提供切实可行的技术方法。方法：Zorbax Sil（4．6mm×150mm，5μm）色谱柱，二氯甲烷-甲醇（99：1）为流动相，流速0．5mL·min^-1，ELSD检测器，漂移管温度100℃，氮气流速3．0L·min^-1。结果：δ-香树酯酮在2．12～10．6μg呈良好的线性关系，药材中该成分的平均回收率为100．5％。结论：该方法简便、准确、分离效果好，无干扰，能准确测定垂盆草中δ-香树酯酮的含量，为制订合理的中药垂盆草的质量标准提供了新的思路。

Objective：To establish the quantitative analysis method of triterpene δ-amyrone in Sedum sarmentosum by HPLC-ELSD. Method： The Zorbax Sil Column was used and the mobile phase was dichloromethane-methanol （99：1 ）. The flow rate was set at 0.5 mL·min^-1, the temperature of drift tube was set at 100℃ and the nebulizer nitrogen flow rate was 3.0 L·min^-1 Result： The linear range of δ-amyrone was 2.12-10.6 μg. The average recovery was 100.5%. Conclution： The results showed that the method was reliable, simple and precise. It would be beneficial for the study of the quality control of S. sarmentosum.