白花败酱草化学成分研究

Studies on chemical constituents of Patrinia villosa

目的∶研究白花败酱草的化学成分。方法:硅胶柱和制备液相色谱分离纯化,理化常数和波谱分析(1H-NMR,13C-NMR,MS)确定结构。结果:从白花败酱草醋酸乙酯和正丁醇萃取部位共分得了7个化合物,分别鉴定为5-羟基-7,3′,4′-三甲氧基黄酮(Ⅰ),5-羟基-7,4′-二甲氧基黄酮(Ⅱ),木犀草素(Ⅲ),槲皮素(Ⅳ),异荭草苷(Ⅴ),异牡荆苷(Ⅵ),8-C-葡萄糖基-7-甲氧基-4′,5-二羟基黄酮(Ⅶ)。结论:Ⅰ,Ⅱ,Ⅲ,Ⅴ,Ⅵ,Ⅷ均是首次从败酱属植物中分离得到,Ⅳ是首次从白花败酱草中分离得到。

Objective： To investigate the chemical constituents of Patrinia villosa. Method： The chemical constituents were isolated by silica gel column chromatography and semi-preparative high-performance liquid chromatography, and identified by physicochemical properties and spectral analysis （MS,^1H-NMR and ^13C-NMR）. Result： Seven compounds were isolated from ethyl acetate and n-butanol extract and identified as： 5-hydroxyl-7, 3＇, 4＇-trimethoxy flavone （ Ⅰ ）, 5-hydroxyl-7, 4＇-dlmethoxy flavone （Ⅱ）, luteolin （Ⅲ）, quercetin （Ⅳ）, isoorientin （ Ⅴ ）, isovitexin （ Ⅵ ） and 8-C glucosylprunetin （ Ⅶ ）. Conclusion： Compounds Ⅰ , Ⅱ, Ⅲ, Ⅴ, Ⅵ and Ⅶ were obtained from the plant of genus Patrinia for the first time, compound Ⅳwas separated from P. villosa for the first time.