皂角刺抑制小鼠宫颈癌U14的生长及对增殖细胞核抗原和p53表达的影响

Effect of Gleditsia sinensis stings on growth inhibition and expression of PCNA and p53 in mice bearing uterine cervical carcinoma(U14)

目的:研究皂角刺醇提物对小鼠宫颈癌U14生长的抑制作用及对增殖细胞核抗原(PCNA)和突变型p53蛋白表达的影响。方法:观察皂角刺醇提物对U14实体瘤和腹水瘤的抑瘤率和生命延长率,并用免疫组化SP法检测PCNA和突变型p53蛋白阳性细胞表达数。结果:以阴性对照组为基数,皂角刺醇提物3个剂量组及环磷酰胺(CTX)阳性对照组实体瘤各组抑瘤率分别为47.44%,59.49%,63.92%,73.42%(P<0.01);腹水瘤各组生命延长率分别为52.21%,67.26%,78.76%,95.58%(P<0.01)。同时各组药物对PCNA和突变型p53蛋白的表达水平也有不同程度抑制作用(P<0.01,P<0.05)。结论:皂角刺对小鼠宫颈癌U14的生长有一定的抑制作用,其机制可能与抑制PCNA和突变型p53蛋白的表达有关。

Objective： To study the antitumor effect of the stings of Gleditsiasinensis on mice bearing uterine cervical carcinoma （U14） and the expression of PCNA （proliferating cell nuclear antigen） and p53. Method： The effect of the ethanolic extract of G. sinensis stings on the inhibition rate of solid tumor and the life span of ascites tumor were calculated by the animal tumor model experiment in vivo. The positive cell numbers of PCNA and mutant p53 protein were measured by immunohistochemical SP method. Result： As compared with the control group, the ethanolic extract of G. sinensis stings （250,500 and 1 000 mg·kg^- 1 body weight, p.o. ） and CTX （25 mg kg^- 1 body weight, i. p. ） administration significantly reduced the tumor weight of solid tumor and increased the life span of ascites tumor harboring mice （ P 〈 0.01）. The inhibition rote of solid tumor and the rote in life span were up to 47.44%, 59.49%, 63.92%, 73.42% and 52.21%, 67.26%, 78.76%, 95.58% respectively. Meanwhile,the expression of PCNA and mutant p53 protein also suppressed by ethanohc extract （ P 〈0.05, P 〈 0.01）. Conclusion： The stings of G. sinensis showed antitumor activity and its possible mechanism might be related with the expression inhibition of PCNA and mutant p53 protein.