非洲紫罗兰叶片再生体系的建立

The Establishment of Regeneration System from Leaves of Saintpaulia ionatha wendl

以非洲紫罗兰幼嫩叶片为外殖体，以MS为基本培养基，添加不同浓度6-BA、NAA、GA等，探讨了外源激素对其愈伤形成、不定芽分化、增殖与不定根形成的影响。试验表明，采用MS＋6-BA0．1～2．0mg／L（单位下同）＋NAA0．01～0．5培养基，愈伤诱导率在20％以上；采用MS＋6-BA0．1～1．0＋NAA0.01～0.5＋GA0．1～1.0培养基，对芽的生长及增殖效果好，并指出GA有提高不定芽长势和成苗率的作用；采用1／2MS＋NAA0．1～0．5或IBA0．1或IAA0．1～0．5，20d内生根率可达97%以上。在腐叶土：珍珠岩=5：3的基质中驯苗，成活率可达90％以上。

Saintpaulia ionatka wendl tender leaves were used as the explant and MS was used as the basic medium and were done in6-BA,NAA,GA of different density. Exploring the effects of external hormone on callus forming uncertain buds differentiation multiplication and uncertain roots forming. The results showed ,in the medium of MS＋6-BA0. 1～2.0 mg/L ＋NAA0.01～0. 5 mg/L, the callus rate is more than 20%, in MS＋6 BA0. 1～1. 0＋NAA0. 01～0. 5＋GA0. 1～1. 0,the effect on multipcation and growth of sprouts is good and point out using GA can promcte indefinite bud growing and survival rate, in 1/2MS＋NAA0. 1～0.5 or IBA0. 1 or IAA0. 1～0.5,which makes the rooting rate reach 97% during 20 days. If the sprouts are transplanted into the substrate in which the rate among humus soil and perlite is 5 ： 3, the survival can over take 90%.