乳腺癌患者血清血管内皮生长因子水平检测

Level of serum vascular endothelial growth factor in patients with breast cancer

目的测定乳腺癌患者血清血管内皮生长因子(vascular endothelial growth factor,VEGF)水平,探讨手术对乳腺癌患者血清VEGF水平的影响。方法采用酶联免疫吸附试验法(ELISA)检测32例正常人和58例乳腺癌患者手术前血清VEGF水平,并比较40例乳腺癌患者手术前后血清VEGF水平。结果血清VEGF浓度与乳腺癌患者临床分期有关,与乳腺癌患者年龄、病理类型无相关;乳腺癌患者血清VEGF水平明显高于正常对照组(P<0.01);Ⅳ期乳腺癌血清VEGF水平明显高于其他各期(P<0.01),Ⅲ期明显高于Ⅱ期(P<0.01),Ⅱ期明显高于Ⅰ期(P<0.01);乳腺癌患者术后血清VEGF水平明显低于术前(P<0.01)。结论血清VEGF可能与乳腺癌的发生发展有一定相关关系,血清VEGF检测对乳腺癌患者病情判断、疗效和预后评价有一定的临床意义。

Objective To detect the level of serum vascular endothelial growth factor（VEGF）in patients with breast cancer and effect of operation on it, and to investigate its clinical value. Methods Enzyme linked immunosorbent assay（ELISA） was used to detect the level of serum VEGF in 32 cases of normal control subject and 58 cases of patients with breast cancer, compared with 40 patients with breast cancer before and after operation. Results Serum VEGF was correlated with clinical stage, and not with age and pathologic types; level of serum VEGF were elevated in all subgroups of patients with breast cancer compared to the controis （P 〈0.01）, the senrum VEGF level in stage IV was higher in comparison with stage Ⅲ and Ⅱ （P 〈0.01）, in stage Ⅲ it was higher than in stage 11 （P 〈0.01）,and in stage Ⅱ it was higher than in stage Ⅰ （P 〈0.O1）;the level of serum VEGF was obviously reduced in patients with breast cancer alter operation（P 〈O.O1）. Conclusions These results suggested that serum VEGF may be involved in the occun＇ence and development of breast cancer, it may be act as an index to assess the disease status and the prognosis of breast cancer.