左旋肉碱对顺铂损伤大鼠肾的保护作用

Protective effects of L-Carnitine on the kidney damage induced by cisplatin in rats

目的:观察左旋肉碱(LC)对顺铂损伤大鼠肾的影响。方法:36只成年雌性W istar大鼠随机分为正常对照组(Control组)、化疗模型组(DDP组)和干预组(LC+DDP组)。Control组和DDP组给予常规饲料,LC+DDP组给予常规饲料+1%LC,喂饲7天后,DDP组和LC+DDP组腹腔内注射顺铂5 mg/kg,Control组腹腔内注射等渗盐水。4天后取标本,测血清肌酐(Cr)、尿素氮(BUN)、肾组织丙二醛(MDA)和谷胱甘肽(GSH)含量,并观察肾的形态结构改变。结果:LC+DDP组与DDP组相比,血清Cr、BUN降低,肾组织MDA含量下降,GSH含量升高,差异显著(P<0.05)或非常显著(P<0.01);病理切片显示肾结构较DDP组有明显改善。结论:预防性喂饲LC能对顺铂损伤大鼠肾产生一定的保护作用。

Objective： To study the protective effects of L-Carnitine（LC） against the kidney damage induced by cisplatin（DDP）. Methods： 36 Female adult Wistar rats were randomly divided into Control group, DDP group and LC ＋ DDP group. Control group and DDP group were fed a basic diet, while LC ＋ DDP group were added 1% LC. After 7 days, DDP group and LC ＋ DDP group received cisplatin i. p. （5 mg/kg body weight）, while Control group received normal saline i.p. After 4 days, rats were killed, the change of serum Cr and BUN, and tissue MDA and GSH in kidney were measured and the kidney structure was also observed. Results： Compared with DDP group, the contents of serum Cr,BUN and kidney tissue MDA of LC ＋ DDP group declined very significantly, while the contents of kidney tissue GSH increased significantly. The pathological slice indicated that the renal structure of LC ＋ DDP group was significantly improved. Conclusion： Dietary supplementation with LC might protect kidney against the damage induced by cisplatin in some extent, which might be partially correlative with the antioxidative effects of LC.