小鼠放射性免疫低下模型的研究

Study on immunorepressive mice model by gamma irradiation

目的保健食品功能性评价中放射性免疫低下模型建立的研究。方法采用30～34g的健康雄性昆明种小鼠90只,随机分成6个组,每组15只,包括3个照射组和3个相应的对照组。对照射组分别以3Gy、4Gy、5Gy剂量γ射线一次性全身照射后,观察照射后3、7、14、21天,小鼠细胞免疫(迟发型变态反应)、体液免疫(血清溶血试验)、单核巨噬细胞系统(鸡红细胞吞噬试验)、NK细胞活性试验等四个方面的变化情况。结果与结论(1)一次性全身3Gy、4Gy、5Gy照射后,在一定的时间范围内,小鼠的免疫系统处于免疫低下状态。(2)不同免疫指标损伤开始恢复的时间不同。血清溶血素对辐射损伤最敏感且损伤恢复最慢,至辐照后21天尚未恢复,腹腔巨噬细胞对辐射损伤恢复最快,在辐照后7d即恢复并代偿性增强。(3)经3Gy、4Gy、5Gy一次性全身照射后3d,体重增长值、脏器指数、足趾肿胀度、血中半数溶血值、腹腔巨噬细胞鸡红细胞吞噬指数均处于低下状态,可以建立放射性免疫低下模型,以评价保健食品的对辐射损伤具有辅助保护功能、免疫调节功能和减低放疗毒副作用的动物模型。(4)脾NK细胞活性指标不适于放射性免疫低下模型的建立。

Objectlve To establish the immunorepressive mice model by irradiation. Methods The 90 Kunming mice which the weight is from 30 to 34g were treated with 3Gy, 4Gy, 5Gy gamma irradiation, the delayed allergy testing, the serum haemolysin level testing, the phagoeytosing functions testing and the NK cell activity testing were performed at 3th, 7th, 14th, 21th days after irradiation respectively. Results and Conclusion （1）All 3Gy, 4Gy, 5Gy gamma irradiation can cause mice immunorepressivestate except the NK cell activity. （2） The time of comeback after irradiation is different from immunity items. The serum haemolysin level is most sensitivity in all, but it comeback most slowly after irradiation. The phagocytosing functions comeback most rapidly after irradiation. （3）It suggests＇ that the irradiation immunorepressive mice model can be established using it in order to evaluate the enhaneeing immune function, assisting irradiation hazard protection function and alleviating toxicity function of irradiation treatment and chemical treatment. （4）the NK cell activity is not suitably to immunorespressive by gamma irradiation.