摘要
将恒电流电解法与流动注射技术相结合,在线电解定量产生不稳定化学发光试剂Mn(Ⅲ),基于Mn(Ⅲ)能直接氧化色氨酸产生化学发光,建立了流动注射电化学发光测定色氨酸的新方法.在优化的实验条件下,测定色氨酸的范围为1.0×10-6～1.0×10-4g/mL,相关系数为0.9996,方法的检出限为3.0×10-7g/mL,相对标准偏差r=2.2%(1.0×10-5g/mL,n=11).
A novel flow injection chemiluminescence method for the determination of tryptophane based on a chemiluminescence reaction between tryptophane and Mn（Ⅲ） was reported. The Mn（Ⅲ） was generated from the solution of MnSO4 by constant current electrolysis on-line. The linear range for the determination of tryptophane is from 1×10^-6 to 1×10^-4g/mL with detection limit of 3×10^-7g/mL. The relative standard deviation was 2.2%.
出处
《石河子大学学报（自然科学版）》
CAS
2005年第6期673-675,共3页
Journal of Shihezi University(Natural Science)
关键词
锰(Ⅲ)
电解
电化学发光
流动注射分析
色氨酸
Mn（Ⅲ）
electrolysis
electrochemiluminescence
flow injection analysis
tryptophane
