摘要
选取36只健康、体况一致的新疆细毛羊随机分成6组。在每只羊的左侧肩胛部皮下局部注射0.5mL(10ng/mL)的IGF-1,右侧肩胛部皮肤为未注射IGF-1的对照组,然后分别于第0、3、6、9、12、50d采集各皮肤样品,用反转录多聚酶链式反应(RT-PCR)方法,定量分析绵羊皮肤中生长激素受体(GHR)、胰岛素样生长因子1(IGF-1)和胰岛素样生长因子1型受体(IGF-1R)、角蛋白关联蛋白KAP3.2和KAP6-1mRNA的相对丰度。试验结果表明,IGF-1对GHR基因的表达有下调的作用,对IGF-1I、GF-1R基因的表达没有显著的影响,而对KAP3.2、KAP6-1基因的表达具有显著的促进作用。说明IGF-1促进绵羊角蛋白关联蛋白基因的表达可能是通过生长轴以外的其他途径实现的。
Thirty-six healthy and similar Xinjiang sheep were selected and divided into six groups at random. Each animal was injected intradermally with 0.5mL IGF-1 (10ng/mL)at left pro-scapulae region, while the right pro-scapulae skin was noninjected control. Treatment and control skin tissue of sheep were. sampled respectively for 0,3,6,9, 12 and 50 days and the skin expression of growth hormone receptor(GHR), insulin-like growth factor 1 (IGF-1 ), insulin-like growth factor receptor 1(IGF-1R),KAP3.2 and KAP6-1 mRNA were measured by RT-PCR. The results indicated that IGF-1 could degrade GHR gene expression, have no effect of IGF-1 and IGF-1R gene expression, and increase significantly KAP3.2 and KAP6-1 gene expression. Taken together these findings that the improvement of KAP3.2 and KAP6-1 gene expression may correlate with other pathway beyond the insulin-like growth factor axis.
出处
《石河子大学学报(自然科学版)》
CAS
2005年第6期690-693,共4页
Journal of Shihezi University(Natural Science)
关键词
绵羊皮肤
相关基因表达
IGF-1
sheepskin
related gene expression
insulin-like growth factor 1