摘要
The effectiveness of thidiazuron (TDZ) on adventitious bud and shoot formation from leaves of Robinia pseudoacacia was tested in combination with naphthaleneacetic acid (NAA) or 2, 4- dichlorophenoxyacetic acid (2, 4-D). With the increasing of TDZ level, the induction rate of adventitious buds appeared an increasing trend and the highest rate was obtained by the combination of 2 mg/L TDZ with 0.1 mg/L NAA.The excessive level of TDZ could induce useless cellae and easily produce hyperhydricity. Inhibition of shoot elongation by TDZ was overcome through transferring shoot cultures to a shoot proliferation medium lacking TDZ or containing NAA, benzyladenine (BA) and TDZ. Indole- 3-butyric acid (IBA) was used to stimulate the rooting in vitro. Based on the number and length of rooting, the treatment with 0.5 mg/L IBA was the best.
The effectiveness of thidiazuron(TDZ)on adventitious bud and shoot formation from leaves of Robinia pseudoacacia was tested in combination with naphthaleneacetic acid (NAA)or 2,4-dichiorophenoxyacetic acid (2,4-D),With the increasing of TDZ level,the induction rate of adventitious buds appeared an increasing trend and the highest rate was obtained by the combination of 2mg/L TDZ whth 0.1mg/L NAA.The excessive level of TDZ could induce useless cellae and easily produce hyperhydricity.Inhibition of shoot elongation by TDZ was overcome through transferring shoot cultures to a shoot proliferation medium lacking TDZ orcontaining NAA,benzyladenine (BA)and TDZ.Indole-3-butyric acid (IBA)was used to stimulate the rooting in vitro .Based on the number and length of rooting ,the treatment with 0.5mg/L IBA was the best.
基金
Supported by project of the Science and technology Ministry of China (Grant No.02 EFN216700794)
关键词
黑洋槐
外芽
TDZ
酪酸
adventitious buds,thidiazuron,black locust ,Robinia pseudoacacia L.