摘要
为检测食品中的丙烯酰胺,建立了饼干、面包、油条、薯片、咖啡多种食品中丙烯酰胺的测定方法。试样经水溶液振荡提取,硅藻土柱净化,采用HPLCMSMS和同位素稀释定量技术,以选择反应监测(SRM)方式测定目标化合物。方法的检出限和定量限分别为4μgkg和12μgkg,线性范围10～3000μgL,不同食品基质的不同加标水平(20～2000μgkg)的回收率为90%～105%,RSD<10%。本方法定量准确、可靠,适用于食品中丙烯酰胺的测定。
A method for determination of acrylamide in foods such as chips, crisps, coffee and some other fried or baked starchrich foods was developed. After adding ^13C3 labeled acrylamide the sample was extracted by water and then cleaned-up by ExtrelutTM 20. Finally the extract was analyzed by HPLC-MS/MS in SRM mode. LOD （limit of detection） and LOQ （limit of quantitation） were 4 μg/kg and 12 μg/kg respectively. Average recoveries for different matrices were 90%- 105% with RSD 〈 10%. The results showed that the method was suitable for determination of acrylamide in chips, crisps, coffee and some other fried or baked starch-rich food.
出处
《中国食品卫生杂志》
2006年第1期5-9,共5页
Chinese Journal of Food Hygiene
基金
国家自然科学基金(30371220)
国家科技重大专项<食品安全关键技术>重要有机污染物的痕量和超痕量检测技术(2001BA804A19和2001BA804A45)
