复方黄柏颗粒剂在小鼠体内的药代动力学

Pharmacokinetics of Compound HuangBo Granule in Mice

为探讨复方黄柏颗粒剂有效成分-盐酸小檗碱在小鼠体内的药动学特征,按25 g/kg(相当于盐酸小檗碱10.95 mg/kg)的剂量经口给予小鼠复方黄柏颗粒剂,用高效液相色谱法检测用药后不同时间血浆中盐酸小檗碱的质量浓度,研究在小白鼠体内的药物动力学。结果表明口服复方黄柏颗粒的有效成分-盐酸小檗碱在小鼠体内的药时数据符合开放性二室模型,动力学方程为C=17.8e-0.859 81t+14.51e-0.636 1t。主要药代动力学参数:t1/2α为(0.81±0.07)h,t1/2β为(5.25±2.96)h,t1/2Ka为(0.76±0.05)h,AUC0→∞为(3.389 3±0.437 4)mg.L-1.h,CL/F为(3.281 8±0.446 3)L/h,Vd/F为(13.65±22.38)L/kg,Tpeak为(1.617 19±0.056 50)h,Cmax为(1.149 98±0.056 04)μg/mL。

To investigate the pharmacokinetics of Compound H uangBo Granule in mice, the effective component--berberine was detected as a representative index. Pharmacokinetics of orally administration of Compound HuangBo Granule at a dose of 25 g/kg （10.95 mg/kg berberine） were investigated . Serum concentration of berberine were measured using HPLC and concentration-time data were analyzed with pharmaceutical kinetics software. The results showed that the ser- um profiles of the drug＇s effective component--berberine were best described by a two compart- ment open model, the pharmacokinetics equation is C=17.8e^-0.85981t＋14.5le^-8.3831t.The main parameters were as follows：t1/2α（0.81±0.07）h,t1/2β（5.25±2.96）h,t1/2Ks（0.76±0.05）h,AUC0→∞（3.3893±0.4374）mg·L^-1·h,CL/F（3.2818±0.4463）U/h,Vn/F（13.65±22.38）L/kg,Tptok（1.61719±0.05650）h,Cmax（1.14998±0.05604）μg/mL.