肉桂挥发油GC特征指纹图谱研究

Study on GC Fingerprinting of Volatile Oils in Cinnamomum cassia

目的建立肉桂挥发油特征指纹图谱,为肉桂的内在质量评价和鉴定提供特征数据。方法采用GC色谱指纹图谱的方法,以广东、广西等不同产地以及基地栽培肉桂为基准,对其挥发油进行主成分比较分析,从中提取主要共有峰并进行相似度计算。色谱条件:HP-5石英毛细管色谱柱(Crosslinked Mehyl siloxame,30m×0．321 mm,0．25 μm);柱温100℃,程序升温以20 ℃·min-1升至140℃,再以2℃·min-1升至200℃;进样口温度240℃,检测器(FID)温度300℃,流速为0．4 mL·min-1,分流比为50:l。结果从规范化种植基地以及广东、广西等不同产地样品GC指纹图谱中归纳出11个主要共有峰,构成肉桂挥发油特征指纹图谱,并对各样品相似度进行比较。结论建立的肉桂挥发油指纹图谱,方法准确可靠,专属性强,特征明显,相似度评价科学客观,可作为肉桂质量控制和鉴别的依据。

OBJECTIVE To establish a fingerprint analysis on the volatile oil of Cinnamomum cassia Presl and to offer the characteristic data for the quality evaluation. METHODS The main components of the volatile oil of Cinnamomum cassia Presl from different producing areas in Guangdong province and Guangxi province were compared by GC fingerprinting. The common components and the resemblance were determined. The GC system consisted of HP 6890 and FID. The analysis was performed on a HP-5 column （ Crosslinked Mehyl siloxame, 30 m ×0.321mm,0.25μm）. The temperatures of sample vent and FID were 240 - 300 ℃ ,respectively. The column temperature was programmedly elevated from 100 - 200 ℃ . The cartier gas was N2. The flow rate was 0.4 mL· min^- 1 . The refractive ratio of distillation was 50 ： 1. RESULTS The GC fingerprinting was established. The similarity degree of the crude drugs from different producing areas were compared. CONCLUSION The method is reliable, accurate and can be applied to the quality control of Cinnamomum cassia Presl.