血小板细胞膜固相色谱法在脉络宁注射液效应物质分析中的应用

Analysis of Active Components in Mailuoning Injection by Platelet Membrane Immobilized Chromatography

目的应用血小板细胞膜固相色谱法分析脉络宁注射液的活性血小板作用成分。方法应用血小板细胞膜作为固定相选择性地结合脉络宁注射液中的活性成分,排除未与血小板靶点结合的非活性成分后,使细胞膜靶点失活,被结合的活性成分从血小板膜上释放出来,样品经固相萃取处理后用HPLC对这些成分进行分析,利用制备色谱得到筛选出的活性成分。结果脉络宁注射液的效应成分初步确定为8个。结论血小板细胞膜固相色谱法可以进行抗血栓栓塞性疾病血管内给药制剂活性成分的筛选,同时反映了化合物与细胞膜生物靶点(受体、通道、酶等)的作用强度。

OBJECTIVE To analysis the effective chemicals in Mailuoning injection by platelet membrane immobilized chromatograghy （PMIC）. METHODS The effective chemicals in Mailuoning injection were combined by the platelet membrane under imitated physical environments. The unattached substances were washed away by the PBS of pH 7.4. Then the attached compounds were eluted by the PBS of pH 4.0. The released compounds from target were collected and analyzed by HPLC.RESULTS Eight characteristic active compounds in the Mailuoning injection were found to bind to the membrane of platelets. CONCLUSION PMIC can be a screening model for the preparations of anti-thromboembolic disease, it can reflect the intensity of the pharmacological action between the compounds and the bMngical targets in the cell membrane, such as receptors, channels and enzymes etc.