摘要
本文通过５种不同抗冻剂，５５组不同浓度组合，筛选出１１组冷冻时能形成玻璃化最低浓度的抗冻剂和１０组解冻时能形成玻璃化的抗冻剂。并对１１组玻璃化液在低温下的一些物理特性进行了测定。不同组合的玻璃化液其冰点和融点各不相同，最高冰点在－１９．５℃，最低达－３９℃。融点最高－４０℃，最低－９０℃。鳝鱼胚胎在５－ｅ中心跳时间最长达３０分钟，在１０－ｃ、５－ｅ中换１７号解冻液后存活最多，达８９．０％以上。
The effects of vitrlfication solution on the survival of sliver carp embryo were studied in this paper.The physical characters of 11 different composition of vitrification solution were determined.The freezing Point of deferent vitrification solution ranged from-19.5℃--39.0℃.The thawing Point of deferent vitrification solution ranged from-40.0℃--90.0℃.The survival rate of silver carp embryo in 10-c and 5-e vitrification solution may exceed 89.0%.
出处
《淡水渔业》
CSCD
北大核心
1996年第5期7-10,共4页
Freshwater Fisheries
关键词
玻璃化液
抗冻剂
成活率
鲢鱼
胚胎
冷冻保存
Vitrification solution,Cryoportectant,Cryopreservation,Survival rate,Silver carp embryo
