噬菌体技术在结核分枝杆菌快速检测中的应用

Application of Rapid Detection for Mycobacterin Tuberculosis with Bacteriophage Splitting Assay

目的评价噬菌体技术在结核分枝杆菌快速检测中的应用价值。方法应用噬菌体技术、ESP培养及涂片法同时检测224份临床确诊为肺结核患者的痰标本。结果①224份标本中噬菌体法、ESP法和涂片法检出的阳性率分别是55.4%,51.7%和27.7%,噬菌体法与涂片法比较有显著性差异(2χ=54.91,P<0.005)。②噬菌体法与涂片法阳性符合率为93.5%(58/62),阴性符合率为59.3%(96/162),总体符合率为68.8%(154/224);噬菌体法与ESP法阳性符合率为82.8%(96/116),阴性符合率为74.1%(80/108),总体符合率为78.6%(176/224)。③在162例涂片法阴性的标本中,噬菌体法检出的阳性率为40.7%(66/162),ESP法检出的阳性率为33.3%(53/162),二者比较有显著性差异(χ2=3.93,P<0.05)。结论噬菌体法对结核分枝杆菌的检出率高于涂片法,对痰涂片为阴性的标本,其检出率高于ESP法,且该方法简便、快速。

Objective This study evaluated the significance of rapid detection for Mycobacterin tuberculosis with Bacteriophage splitting assay. Methods 224 clinical sputum specimen from the patients of the pulmonary tuberculosis were detected by Bacteriophage splitting,ESP and smears test. Results ①Among 224 samples of sputum specimen,the positive rate with Bacteriophage splitting,ESP and smears test were 55. 4%, 51.7 % and 27.7%. The positive rate with Bacteriophage splitting was significant higher than that of smears tests （x^2= 54.91 ,P〈0. 005）. ②In Bacteriophage splitting and smears test,there were 93.5% （58/62） tally with masculine-rate,59.3% （96/162） tally with negative-rate and 68.8%（154/224） tally with both. In Bacteriophage splitting and ESP,there were 82.8%（96/116） tally with masculine-rate,74.1% （80/108） tally with negative-rate and 78.6% （176/224） tally with both. ③Among the 162 sample of negative tuberculosis smear,40. 7%（66/162） were tested positive in Bacteriophage splitting where as only 33.3%（53/162） were tested positive in ESP. t Statistically significant difference was found in both of contrastive tests （x^2=3.93,P〈0.05）. Conclusion The Bacterio- phage splitting assay can be used for rapid identification of Mycobacterin tuberculosis. Compared with ESP,Bacteriophage splitting can test bacillus more efficiently and quickly in negative tuberculosis smear.