摘要
在硼酸缓冲溶液(pH 6.7)介质中,H2O2对鲁米诺的电化学发光有敏化作用,而褪黑素(MLT)因具有对自由基的湮灭作用而对此敏化的电化学发光具有淬灭作用,该发光体系的ECL光强与MLT的浓度成线性关系,检测灵敏度极高,检测下限可达到1×10-14mol/L,用于脑白金胶囊中MLT的测定得到满意结果,为MLT的测定提供了一种灵敏、可靠的测定方法。
In the borate buffer solution of pH 6.7, the melatonin（MLT） quenched the H2O2 enhancing electrochemiluminescense（ECL） of luminol because of the annihilation to the oxygen free radicals. It may be sensitively determined by this method. The ECL luminous intensity linearly decreases with increase of the concentration of MLT, the detection limit is 1×10^-14mol/L, and the capsule samples have been determined with satisfactory results. It should be a sensitive and reliable method for the determination of melatonin.
出处
《分析试验室》
CAS
CSCD
北大核心
2006年第1期17-19,共3页
Chinese Journal of Analysis Laboratory
基金
国家自然科学基金资助项目(20275025)
