流动注射化学发光分析法测定三类磺胺类药物

Flow injection-chemiluminescence determination of three classes of sulfa drugs

研究发现可溶性锰（Ⅳ）氧化磺胺类药物（磺胺甲基异唯唑、磺胺嘧啶和磺胺脒）可以产生弱的化学发光，甲醛对这一化学发光反应有较强的增敏作用，据此建立了一种测定磺胺类药物的流动注射化学发光分析法。该方法对3种磺胺类药物磺胺甲基异皓唑、磺胺嘧啶、磺胺脒的检出限分别为2×10^-8、3×10^-8、2×10^-8g／mL；线性范围分别为6．0×10^-8～1．0×10^-5、1．0×10^-7～8．0×10^-6、4．0×10^-8～8．0×10^-6g／mL。对6．0×10^-6g／mL磺胺甲基异口恶唑、4．0×10^-6g／mL磺胺嘧啶和4．0×10^-6g／mL磺胺脒的相对标准偏差分别为1．1％、1．2％和2．2％（n=11）。此法已用于复方新诺明片剂中磺胺甲基异口恶唑的测定，结果与药典方法测定值一致。并对化学发光反应的机理进行了探讨。

Based on formaldehyde enhancing the chemiluminescence in the oxidation of sulfa drugs by soluble manganese （ Ⅳ ）, a new chemiluminescence method to determine sulfa drugs such as sulfamethoxazole, sulfadiazine and sulfaguannidine with flow injection technique was developed. Under optimum conditions, the linear ranges for the determination of sulfamethoxazole, sulfadiazine and sulfaguannidine were 6.0×10^-8～1.0×10^-5g/mL、1.0×10^-7～8.0×10^-6g/mL and 4.0×10^-8～8.0×10^-6g/mL with the limits of detection of 2×10^-8g/mL、3×10^-8 and 2×10^-8g/mL, respectively. The relative standard deviations for the determination of 6.0 ×10^-6 g/mL sulfamethoxazole, 4.0 × 10^-6 g/mL sulfadiazine and 4.0 × 10^-6 g/mL sulfaguannidine were 1 . 1%, 1.2% and 2.2% （ n = 11 ）, respectively. The method has been applied to the determination of sulfamethoazole in compound sulfamethoxazole tablets and the results were in good agreement with those obtained by pharmacopoeia method. The preliminary mechanism of the reactions was also investigated.