摘要
小鹅瘟强毒和鸭胚化弱毒可以在产蛋母鸡中增殖,并传递到卵黄中。卵黄中的病毒抗原可以用琼脂扩散试验和对流免疫电泳检出,该沉淀线可被高免血清阻断。在人工感染产蛋母鸡45天后,仍能在感染母鸡所产蛋的卵黄中检到小地瘟病毒抗原。含病毒的鸡蛋在普通冰箱中保存3个月,仍可用琼扩试验检测出卵黄中病毒抗原。该试验为小鹅瘟病毒的增殖提供了一种途径。
Goose parvovirus GPV strain SRM1 and GD can reproduce in hen, and also Can be tramsmited to yolk,GPV antigen in yolk is detectible by agar gel immunodiffusion(AGlD) test and counter immunoeletrophoresis (CIE). The specific precipitation line between GPV antigen and tested serum can be withdrawn when GPV antigen is neutralized by hynerim-mune serum. GPV antigen can be detected in yolk even the hen was infected45 days before the eggs were produced. The antigen can still be rereled in the yolk after kept in refrigerator for three months. A new method of propagating GPV is Demonstrated by the Study.
