肠球菌抗生素耐药基因检测

Detection on Antibiotics-resistant Genes in Enterococci

目的明确解放军第98医院临床分离的肠球菌中,抗生素耐药相关基因存在状况。方法采用聚合酶链反应(PCR)及序列分析的方法,分析15株粪肠球菌和9株屎肠球菌中,β-内酰胺类抗生素耐药相关基因(TEM)、氨基糖苷类抗生素耐药相关基因(氨基糖苷类修饰酶基因)[aac(6′)/aph(2″)、aph(3′)-Ⅲ、ant(2″)-Ⅰ、ant(4′,4″)、ant(6)-Ⅰ]、四环素耐药相关基因(tetM)、红霉素耐药相关基因(ermB、mefA)和万古霉素耐药相关基因(vanA、vanB)。结果24株肠球菌中TEM、aac(6′)/aph(2″)、aph(3′)-Ⅲ、ant(2″)-Ⅰ、ant(4′,4″)、ant(6)-Ⅰ、ermB、mefAt、etM、vanA和vanB 4基因的阳性分别为9株(37.5%)、17株(70.8%)、6株(25.0%)、0、0、10株(41.7%)、18株(75.0%)、0、10株(41.7%)、1株(4.2%)和1株(4.2%)。结论临床分离的肠球菌多重耐药严重;携带抗生素相关耐药基因是导致菌株对抗生素产生耐药的重要原因。

OBJECTIVE To investigate the antibiotics-resistant genes in enterococci isolated from the 98th Hospital of PLA, H uzhou, Zhejiang Province, China. METHODS The antibiotics-resistant genes of TEM, aac （6＇）/aph （2＂）, aph（3＇）-Ⅲ , ant（2＂）-Ⅰ, ant（4＇,4＂）, ant（6）-Ⅰ , ermB, mefA, tetM, vanA, and vanB were analyzed by polymerase chain reaction （PCR） and verified by DNA sequencing in the 15 isolates of Enterococcus faecalis and 9 isolates of E. faecium. RESULTS The positive rate of the resistance genes of TEM, aac（6＇）/aph（2＂） , aph（3’）-Ⅱ , ant（2＂）-Ⅰ , ant（4＇, 4＂）, ant（6）-Ⅰ , ermB, mefA, tetM, vanA, and vanB in the 24 strains of enterococci tested were37.5%, 70.8%, 25.0%, 0.0%, 0.0%, 41. 7%, 75.0%, 0.0%, 41. 7%, 4. 2%, and 4. 2%, respectively. CONCLUSIONS The multidrug resistance of enterococci was a serious issue, and harbored antibiotics-resistance genes were the very important reasons of resistance to antibiotics in enterococci.