茶多酚对乙醇性肝损伤大鼠全血内毒素水平的影响

Tea Polyphenol Influences Blood Plasma Endotoxin Level in Alcohol Liver Hurted Rat Model

目的探讨茶多酚对乙醇性肝损伤大鼠血浆内毒素水平的影响,为乙醇性肝病的预防和治疗提供新的手段和方向。方法S-D大鼠随机分成5组,每组大鼠雌雄各半;每只大鼠分别按7 g/kg剂量,以56度北京产红星二锅头酒,1次/d灌胃;同时,除A组单纯白酒灌胃外,B、C、D组分别以0.05、0.125、0.25 g/kg的剂量,乙醇灌胃同时每天灌胃给予茶多酚;E组为单纯对照组,仅给予以饮用水灌胃1次/d;于4周末,采大鼠股静脉血测定血清ALT、AST水平和以鲎试验测定全血内毒素。结果单纯灌酒组的血清AST水平明显高于其他4组;根据全血内毒素测定结果,发现茶多酚用药组全血内毒素水平低于单纯灌酒组;尤以茶多酚中剂量组(0.125g/kg)更加明显(16.33±9.09 vs91.50±36.83,P<0.01)。结论本试验初步提示了茶多酚具有降低乙醇性肝损伤大鼠全血内毒素水平作用,其可能的机制是抑制肠道菌群异位和维持胃、肠黏膜的正常功能;它为治疗乙醇性肝损伤,提供了一条新的途径。

OBJECTIVE To investigate the influence of tea polyphenol in alcoholic liver hurted rat by observing its blood plasma endotoxin level, and to provide the direction of new treatments and means for the prevention of alcoholic liver disease. METHODS Thirty S-D rats （15 female, 15 male） were divided into five groups randomly （noted as A, B, C, D, and E, with equal male and female）, and fed in the cleanliness class laboratory. Excepting group E which was given saline, all other groups were given alcohol （56% Hongxing spirits） with the dosage of 7 g/kg once everyday. At the same time, rats in groups B, C and D were given tea polyphenol with the dosage of 0.05, 0. 125, 0.25 g/kg. In the end of the fourth week, got the rats vein blood to determinae serum ALT, AST level and blood plasma endotoxin level. RESULTS The serum AST of the group A was higher than other 4 groups; the blood plasma endotoxin level in groups B, C, and D were consequently lower than in group A. CONCLUSIONS The tea polyphenol could reduce the blood plasma endotoxin level in alcoholic liver hurted rat. Its possible mechanism is to repress the isotopy of bowel flora, and to maintain the normal function of bowel mucous membrane. This research provides a new way for curing the alcohol liver hurt.