雷公藤甲素对小鼠巨噬细胞活性和NO分泌的影响

Effect of Triptolide on Cytotoxicity and NO production of Activated Mouse Peritoneal Macrophage

目的：探讨雷公藤甲素（TP）对子宫内膜异位症患者腹腔液中大量激活的巨噬细胞免疫抑制作用机制，以及其在不同剂量TP和不同时间段的杀伤活性和对NO分泌的影响。方法：体外培养由脂多糖（LPS）诱导激活的小鼠腹腔巨噬细胞，采用噻唑盐比色（MTT）间接法观察其杀伤活性的变化，并用硝酸还原酶法检测其上清波NO的含量变化。结果：1～10μg／ml TP对巨噬细胞杀伤活性和NO的产生具有明显抑制作用（P〈0．01），且无细胞毒性。TP〈1μg／ml无统计学意义（P〉0．05）。结论：TP能有效抑制小鼠腹腔液中活化巨噬细胞和NO的生成，为临床治疗子宫内膜并位症提供实验依据。

Objective：To study the mechanism of immune inhibition of triptolide（TP） on activated peritoneal macrophage （PEMΦ） in humane endometriosis and the effect of different dosage of TP on Cytotoxicity and NO production in different period. Methods：The activated PEMΦ induced by LPS was cultured in vitro. The eytotoxicity of MΦ was tested by MTT eolorimetry and the level of NO by Griess reagent. Results：TP with the dosage of 1-10μg/ml could significantly inhibit the eytotoxicity （ P 〈 0. 0 1 ） and NO production of PEMΦ and had no eytotoxicity to PEMΦ. TP with the dosage less than 1μg/ml had no statistic meaning. Conclusion：TP can effiendy inhibit the eytotoxieity and NO production of activated PEMΦ, which provides experimental proof for the clinical treatment of endometriosis.