摘要
p53基因是迄今发现与人类肿瘤相关性最高的抑癌基因,通过克隆p53基因对其表达调控进行研究。利用分子生物学软件GCK2.0对整个克隆过程进行分析,制定克隆策略,通过测序鉴定结果,得到了含目的基因p53cDNA全序列的pTRE-p53重组质粒。通过GCK2.0分析,减少了基因克隆的盲目性,提高了工作效率。
p53 gene is the most consanguineous anticancer interrelated to human cancer. To clone p53 gene and study the fuctions of p53 and its downstream gene. the authors used molecular biology soft Gene Construnction Kit 2.0 （GCK2.0） to analyse the process of gene cloning for the construction strategy of pTRE-p53. Compared with the results of the experiments, the authors found the pTRE-p53 plasmid including p53 cDNA sequence was successfully constructed. The anomic of gene cloning is reduced, and the efficiency of gene cloning is increased as GCK2.0 is used.
出处
《重庆邮电学院学报（自然科学版）》
2006年第1期96-98,共3页
Journal of Chongqing University of Posts and Telecommunications(Natural Sciences Edition)
基金
国家自然科学基金资助项目(30000084)
