摘要
综述了生物样品(主要为粪便、尿液、血液和培养液)中短链脂肪酸的提取与测定方法,讨论了各种样品提取方法的优缺点,并比较了气相色谱、高效液相色谱和毛细管电泳分离检测的优点和局限性。引用文献63篇。
Short-chain fatty acids are organic acids with 1 - 6 carbon atoms. Their physiological functions and clinical applications have attracted considerable attention. Their high polarity, low ultraviolet （UV） absorbance, low contents in biological samples, high volatility, good water-solubility and easy adsorption on metal and glass surfaces result in some difficulties in their separation and determination. This review focuses on the methods for extraction of short- chain fatty acids in biological samples such as feces, urine, blood and culture solutions by distillation, high speed centrifugation, liquid-solid extraction, solid phase microextration, supercritical fluid extraction and liquid phase microextraction and determination by gas chromatography, high performance liquid chromatography and capillary electrophoresis. Sixty-three references are cited.
出处
《色谱》
CAS
CSCD
北大核心
2006年第1期81-87,共7页
Chinese Journal of Chromatography
基金
国家杰出青年科学基金(No.20325518)
国家重点基础研究发展规划(No.2003CB515502)
关键词
短链脂肪酸
生物样品
提取方法
气相色谱
高效液相色谱
毛细管电泳
综述
short-chain fatty acids （SCFA）
biological materials
extraction method
gas chromatography （ GC ）
high performance liquid chromatography （ HPLC ）
capillary electrophoresis （CE）
review
