高碘酸氧化肝素抑制P-选择素介导的A375细胞粘附

Periodate-oxidized Heparin Inhibiting P-selectin-mediated A375 Cell Adhesion

通过选择性化学修饰制备出具有低抗凝血活性的高碘酸氧化肝素,并系统地分析了其抗肿瘤细胞粘附的活性.研究结果表明,具有低抗凝血活性的高碘酸氧化肝素仍然可以显著地抑制P-选择素介导的人恶性黑素瘤A375细胞与血小板间的粘附.

Numerous experiments show that heparin affects tumor metastasis in various ways, and one of the mechanisms by which heparin inhibits metastasis is by blocking the P-selectin-based interaction of platelets with tumor cells. However, heparin＇s significant anticoagulant properties and the potential of bleeding complications may contraindicate its use as an anti-metastasis compound. RO-heparin with low anticoagulant activity was tested as the inhibitor of human malignant melanoma A375 cells adhering to surface-immobilized platelets expressing P-selectin. Then we demonstrate that RO-heparin can block P-selectin-mediated A375 human melanoma cell adhesion, suggesting that it may has a potential value as a anti-metastatic, therapeutic agent.