摘要
利用青霉素和链霉素分别对α-ALDC产生菌枯草芽孢杆菌3226-5、V-20进行抗药性标记,筛选得到稳定的青霉素抗性标记株P-67和链霉素抗性标记株S-17,并以P-67和S-17为融合的亲本对其原生质体融合条件进行了初探。
Regard α-ALDC producting strains BaciLLius subtiLis 3226-5 and V-20 as parents strain, penicillin resistant strain P-67 and streptomycin resistant strains S-17 were abtained. The conditions of fusion were discussed.
出处
《啤酒科技》
CAS
2006年第1期15-18,20,共5页
Beer Science and Technology
基金
河北省重点攻关课题(94245509D)
关键词
抗药性标记
原生质体
融合
Α-乙酰乳酸脱羧酶
resistant strain, protoplast, fusion, α-acetolactate decarboxylase (α-ALDC)