碱性成纤维细胞生长因子基因对缩小猪急性心肌梗死面积的作用观察

Effects of basic fibroblast growth factor gene on the reduction of infarction size in a swine model of acute myocardial infarction

目的探讨经导管冠状动脉内导入碱性成纤维细胞生长因子(bFGF)基因(pcDNA3-bFGF)对缩小猪急性心肌梗死面积的作用。方法实验动物分为两组:手术对照组(n=6),冠脉内bFGF基因导入组(n=6)。开胸结扎猪冠状动脉左旋支(LCX),建立急性心肌梗死动物模型。构建真核表达质粒pcDNA3-bFGF,并经导管冠脉内导入,通过心肌酶学及碱性复红苦味酸法(HBFP)特殊染色观察pcDNA3-bFGF对猪急性心肌梗死面积的影响。结果术后24 h,两组乳酸脱氢酶同工酶(LDH-1)及CK-MB较术前明显升高(P<0.01);术后48 h,bFGF基因导入组LDH-1及CK-MB明显低于手术对照组(P<0.01)。HBFP特殊染色显示,bFGF基因导入组心肌梗死面积明显小于手术对照组(P<0.05)。结论经导管冠状动脉内导入bFGF基因有缩小猪急性心肌梗死面积的作用。

Objective To investigate the effects of basic fibroblast growth factor gene on the reduction of infarction size in a swine model of acute myocardial infarction. Methods All the swines were divided into two groups： operation control group （ n = 6） and intracoronary gene group （ n = 6）. Swine models were made by opening chest and ligating the left circumflex （LCX） coronary artery, peDNA3 - bFC, F eukaryotic expression plasmid was construeted and was directly injected into coronary artery by the catheter to observe the influences on infarction size according to myocardial enzyme and HBFP patho - histological sections. Results 24 hours （h） after operation, the values of LDH- 1 and CK- MB were higher than that of before operation（ P 〈 0.01 ）. 48 h after operation, the values of LDH - 1 and CK- MB in bFGF gene group were lower than those in control group（ P 〈 0.01 ）. In bFGF gene group, infarction size was smaller than that in control group（ P 〈 0.05）. Conclusions bFGF gene injected into coronary artery by the catheter can reduce infarction size in the swine models of acute myocartial infarction.