应用PCR技术检测急性淋巴细胞白血病T细胞受体γ基因重排

Detection of T-Cell Receptor γ Gene Rearrangement in Acute Lymphoblastic Leukemia Using Polymerase Chain Reaction

目的寻找可作为急性淋巴细胞白血病恶性克隆的基因标记。方法应用ＰＣＲ技术扩增Ｔ细胞受体γ链基因重排，选用四种限制性核酸内切酶消化扩增产物。结果初诊或复发１０例急性淋巴细胞白血病病人中，有５例出现清晰扩增带，约４００ｂｐ，扩增物经四种限制性核酸内切酶消化后形成的限制性核酸内切酶图谱各具特色；５例处于缓解期的急性淋巴细胞白血病病人，有４例被检出清晰扩增带。结论应用ＰＣＲ扩术扩增Ｔ细胞受体γ链基因重排，能用于检测急性淋巴细胞白血病微小残留病，如果结合限制性核酸内切酶图谱分析。

To find out a kind of genetic mark of malignant colony in acute lymphoblastic leukemia(ALL).Methods After amplifying T-cell receptorγchain gene rearrangement using polymerase chain reaction(PCR),we digested the products with 4 kinds of restriction enzyme.Results Clear bands were detected from 5 of l0 ALL bone marrow samples at presentation or clinical relapse using this method,the length of the products was about 400 bp.Each of the restriction patterns formed from the digestion of the amplified products by the 4 kinds of restriction enzyme was characteristic.4 of 5 bone marrow samples at complete remission were detected to have clear amplified bands;Conclusion That the amplification of T-cellγchain gene rearrangement using PCR can be used to detect minimal residual disease in ALL,and can be used to research the evolution of leukemic colony if combined with restriction analysis of the PCR products.